3D Vision: The Revolutionary Technology of Volumetric Imaging

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Traditional microscopy provides only two-dimensional static images, much like trying to understand a sculpture from a flat photograph - unable to capture the dynamic three-dimensional information of biological samples. The breakthrough of volumetric imaging technology allows us to instantly record biological activities throughout three-dimensional space, achieving a "live broadcast" of life processes.
Technical Advantages:
  • High-Speed Capture
: Records rapid biological processes like neural signal transmission at millisecond-level speeds
  • Complete 3D Information
: Directly acquires spatial structure of samples and intercellular interactions
  • Reduced Damage
: Significantly lowers phototoxicity and photobleaching, more suitable for long-term live observation
Main Technical Approaches:
  1. Light Sheet Microscopy
: Illuminates samples with thin side-layer light sheets, enabling high-speed, low-damage 3D imaging - an ideal tool for developmental biology research
  1. Structured Illumination
: Uses special grating patterns to break resolution limits, obtaining super-resolution 3D images
  1. Spinning Disk Confocal
: Significantly improves imaging speed while maintaining optical sectioning capability
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Application Fields:
  • 3D mapping of whole-brain neural connections
  • Real-time observation of embryonic development processes
  • Evaluation of organoid drug responses
  • Tracking immune cell migration
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