Traditional microscopy provides only two-dimensional static images, much like trying to understand a sculpture from a flat photograph - unable to capture the dynamic three-dimensional information of biological samples. The breakthrough of volumetric imaging technology allows us to instantly record biological activities throughout three-dimensional space, achieving a "live broadcast" of life processes.
Technical Advantages:
: Records rapid biological processes like neural signal transmission at millisecond-level speeds
: Directly acquires spatial structure of samples and intercellular interactions
: Significantly lowers phototoxicity and photobleaching, more suitable for long-term live observation
Main Technical Approaches:
: Illuminates samples with thin side-layer light sheets, enabling high-speed, low-damage 3D imaging - an ideal tool for developmental biology research
: Uses special grating patterns to break resolution limits, obtaining super-resolution 3D images
: Significantly improves imaging speed while maintaining optical sectioning capability
Application Fields:
- 3D mapping of whole-brain neural connections
- Real-time observation of embryonic development processes
- Evaluation of organoid drug responses
- Tracking immune cell migration