Selection and Matching Recommendations for Microscope Accessories
Created on 09.09
I. Core Optical Accessories: Determine Imaging Quality
1. Eyepieces: Prioritize Comfort and Compatibility
- Field Number (FN): Larger FN means a wider field of view. Choose FN20-25 for scientific research/medical use (suitable for long-term observation) and FN16-18 for education (cost-effective);
- Key Functions: Select eyepieces with ±5 diopter adjustment (for myopic/hyperopic users). Choose trinocular eyepieces (to connect cameras) for documentation needs, and reticle eyepieces for quantitative measurement;
- Pitfall: Avoid blindly pursuing "high-magnification eyepieces" (e.g., 30×). Pairing a 100× objective with a 30× eyepiece may blur images (exceeding the resolution limit of optical microscopes).
2. Objectives: Numerical Aperture (NA) is Core
- Numerical Aperture (NA): Higher NA means stronger resolution. Use a 40× dry objective (NA0.6, for overall cell observation) or a 100× oil-immersion objective (NA1.4, for bacteria/mitochondria—must-have for medical/scientific use);
- Scenario Adaptation: Low magnification (4×-10×) for sample positioning, medium magnification (20×-40×) for cell/tissue observation, high magnification (100×) for fine structures, and ultra-high-pressure objectives (200×-400×) for nano-scale chip detection;
- Correction Types: Achromatic objectives (low-cost) for education, plan-achromatic objectives (uniform clarity at center and edges) for scientific/medical use, and apochromatic objectives for ultra-fine observation;
- Foreign Trade Note: Confirm the main unit’s interface first (e.g., Olympus UIS2, Leica HC). For educational microscopes, choose universal RMS-threaded objectives to avoid installation issues.
II. Sample Preparation Accessories: Ensure Qualified Samples and Efficient Observation
1. Slides and Coverslips
- Materials: Optical glass (transmittance ≥90%, suitable for optical/fluorescence microscopes), non-fluorescent slides (to avoid fluorescence interference in medical/scientific use), and plastic slides (disposable for clinical use, reducing cross-contamination);
- Specifications: Standard 76×26mm (fits most stages, thickness 1.0-1.2mm). Choose large-size slides for industrial large samples or circular slides for cell culture;
- Coverslips: Thickness 0.13-0.17mm (matches high-magnification objectives, scratch-free and bubble-free to prevent stray light).
2. Sample Processing Tools
- Biological Samples: Manual microtomes (5-10μm sections) for education/small batches; automatic microtomes (1-5μm sections, high uniformity) for scientific/medical large batches. Pair with cell culture coverslips and staining jars (to save staining solution);
- Industrial Samples: Metal clamps for hard samples (chips/metals) and rubber clamps for soft samples (plastics/textiles). Use polishing machines for metal/rock samples (to create mirror-like surfaces and avoid scratch-induced imaging issues);
- Powder/Liquid Samples: Neubauer counting chambers for bacteria/cell counting (modified versions for blood cells); quartz cuvettes for fluorescence/UV observation, and glass cuvettes for ordinary liquids (fits stages).
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