In the foreign trade procurement of microscopes, accurately grasping the core parameters is crucial to avoid wrong selection. Magnification, resolution, and numerical aperture need to be considered comprehensively, while auxiliary parameters such as objective lens type, illumination method, and focusing system also affect the user experience. The core points are as follows:
I. Core Performance Parameters: Determine Observation Capability
1. Magnification: Match Requirements, Avoid Blindly Pursuing High Magnification
Calculated by multiplying the magnification of the objective lens and the eyepiece, it should be selected based on the observation target:
- Basic teaching/routine observation: 100-1000x is sufficient;
- Fine detection/scientific research: Over 2000x is required.
Beware of "invalid magnification" – high magnification beyond the support range of resolution will only cause blurry images and have no practical significance.
2. Resolution: Core of "Seeing Details Clearly"
Measured by the minimum distinguishable distance (μm), the smaller the value, the higher the precision:
- Conventional optical microscopes: Approximately 0.2 μm, suitable for biological sections, etc.;
- Electron microscopes: 0.1-0.001 nm, suitable for atomic-level observation.
When purchasing, it is necessary to meet the precision requirements of details and conduct a comprehensive evaluation combined with numerical aperture.
3. Numerical Aperture (NA): Affects Brightness and Resolution
Marked on the objective lens, the larger the value, the stronger the light-gathering ability, image brightness, and resolution (resolution formula: Resolution = 0.61λ/NA):
- Low-magnification observation (4-10x objective lens): 0.1-0.2;
- Medium-high magnification observation (20-40x objective lens): 0.4-0.6;
- High-magnification observation (100x oil-immersion objective lens): ≥0.85.